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hIL18 Effector Reporter Cell

CBP74173

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I. Background
Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.
 
II.Introduction
Expressed gene: hIL18
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): IL18; IGIF; IL-18; IL-1g; IL1F4; interleukin 18
Freeze Medium: 90% FBS+10%DMSO
Culture Medium: RPMI-1640+10%FBS+1ug/ml puromycin+800ug/ml hygromycin+10ug/ml blasticidin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
 
III. Representative Data

Figure 1.Dose Response of Recombinant IL-18 in IL-18 Effector Reporter Cells(C12).

Figure 2.Inhibition of hIL18-Induced Reporter Activity By Recombinant Human IL18 BP Protein in hIL18 Effector Reporter Cells(C12).

 

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