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BT474-Luc

CBP30103L

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I. Introduction
Host Cell: BT-474 HER2 amplification
Expressed gene: Luciferase
Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+0.2%BT474 advance+2ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen
 
II. Description of Host Cell Line
Organism: Homo sapiens,human
Tissue: Mammarygland;breastduct
Disease: Ductal carcinoma
Morphology: Epithelial
Growth Properties: Adherent,patchy The cells form adherent patches of epithelial-like cells The patches are compact multilayered colonies that rarely become confluent
 
III. Representative Data
CBP30103L 信號

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 484±16(Mean±SD) on BT474 cells while 691026±25124(Mean±SD) on BT474-Luc cells.

 
IV. Stablity Test
CBP30103L 穩(wěn)定性

Figure. Stablity test results. BT474-Luc cells showed at least 32 passage stability with similar luminescence unit.


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