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C3aR/CHO

CBP71375

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I. Background
C3aR binds the complement activation fragment, C3a. It is a G-protein coupled receptor of 482 amino acids, notable for its large second extracellular loop. As with the other closely related anaphylatoxin receptors, the genetic locus for this receptor consists of two exons separated by a large intron. The receptor is widely expressed, and in high levels on cells of the myeloid lineage. 
 
II. Introduction
Host Cell:

CHO

Stability: 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: F12K+10%FBS+5ug/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen immediately upon delivery
Application(s):

Functional assay for C3aR

Transducer: Gi
 
Ⅲ. Description of Host Cell Line
Organism: Hamster
Tissue: Ovary
Morphology: Epithelial
Growth Properties: Adherent
 
Ⅳ. Representative Data

Figure 1. Recombinant C3aR/CHO constitutively expressing C3aR.

Figure 2. HTRF cAMP Assay With C3aR CHO Cells (C4).


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