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NCI-H716(結直腸腺癌細胞)

CBP60737

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I. General information 
Synonyms: NCI-H716
Background: Established from the primary tumor of a 33-year-old Caucasian  man with cecum adenocarcinoma in 1984;  
Species: Homo sapiens, human
Tissue: cecum
Disease: Colorectal adenocarcinoma
Gender: male, 33 years  adult,Caucasian
Morphology: epithelial  
Growth Mode: suspension, multicell aggregates and some adherent cells
Doubling Time: 40~60hrs
DNA Profile: Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 11,12
D5S818: 11
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,17
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+10%FBS

NCI-H716完全培養基,# CBP60737M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Tumor Formation: Yes, in nude mice
(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Comments: The cells contain Dopa decarboxylase and, unlike other colorectal lines, contain cytoplasmic dense core granules characteristic of endocrine secretion.
The cells do not express the TAG-72 or CA19-9 antigens nor do they produce carcinoembryonic antigen (CEA).
This line was derived from cells present in ascites fluid obtained from a patient after treatment with 5-fluorouracil.
For more information, please contact Cobioer (4008-750-250).

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