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HCT-15(人結腸癌腫瘤細胞)

CBP60029

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I. General information 
Synonyms: HCT-15
Background: Evidence from DNA fingerprinting indicates that this line and DLD-1 (ATCC CCL-221) are derived from the same individual; however, isoenzymology and cytogenetic data leave some doubt.
Species: Homo sapiens, human
Tissue: colon
Disease: Dukes' type C, colorectal adenocarcinoma
Gender: male
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640 + 10%FBS

HCT-15完全培養基,# CBP60029M

We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Genes Expressed: carcinoembryonic antigen (CEA) 5.4 ng/106 cells/10 days. The cells are positive for keratin by immunoperoxidase staining.
Cellular Products: carcinoembryonic antigen (CEA) 5.4 ng/106 cells/10 days and keratin
Tumor Formation: Yes, in nude mice
(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Comments: HCT-15 cells are CSAp negative (CSAp-).
The cells are positive for keratin by immunoperoxidase staining.
For more information, please contact Cobioer (4008-750-250).

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