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首頁 /藥靶模型 /GPCR靶點(diǎn)細(xì)胞 /Class A(Rhodopsin) /Human ADORA2B Gα15/CHO

Human ADORA2B Gα15/CHO

CBP71019

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I. Background

The adenosine receptors ADORA2B is Gs and Gq/G11-coupled GPCR expressed in the large intestine, cecum, and urinary bladder. ADORA2B receptor mediates relaxation to adenosine in human small coronary arteries which is independent of NO but dependents in part on a K+-sensitive mechanism. Pharmacological or molecular biologic activation of ADORA2B receptor may prevent glomerular remodeling associated with glomerulosclerosis, renal disease, and abnormal growth associated with hypertension and diabetes.

 
II. Introduction
Host Cell:

CHO

Stability: 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: Ham’s F12+10%FBS+400μg/ml G418+100μg/ml Hygromycin B
Mycoplasma Status: Negative
Storage: Liquid nitrogen immediately upon delivery
Application(s):

Functional assay for ADORA2B

Transducer:   Gs
 
Ⅲ. Description of Host Cell Line
Organism: Hamster
Tissue: Ovary
Morphology: Epithelial
Growth Properties: Adherent
 
Ⅳ. Representative Data


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