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BRAF L597R/BaF3

CBP73387

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I. Introduction
Cell Line Name: BRAF L597R/BaF3
Host Cell: Ba/F3
Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application: Anti-proliferation assay and PD assay
Freeze Medium: 90% FBS+10% DMSO
Complete Culture Medium: RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
 
II. Background
BRAF L597R lies within the protein kinase domain of the Braf protein (UniProt.org). L597R results in activation of Braf as indicated by increased phosphorylation of Mek and Erk in cell culture, is associated with Erk activation in a patient tumor sample, and in one of two cell lines, increased cell proliferation and cell viability compared to wild-type Braf.
 
III. Representative Data

1. WB of BRAF L597R/BaF3

2. Sanger of BRAF L597R/BaF3

Figure 2. Sanger of BRAF L597R/BaF3

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of BRAF L597R BaF3 Cells (C3).

 

 

 

 

 

 

 

 

 

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